Ph.D. 1973, Hebrew Univ.
Lect. 1975; Sen. Lect. 1978
Assoc. Prof. 1982
Field of Study
1. Genetic dissection of phototransduction.
2. Ca2+ signaling.
3. The gating mechanism of the light-activated channels TRP and TRPL.
4. Mechanisms underlying hereditary retinal degeneration.
5. Visual transduction.
6. Light-activated translocation of signaling molecules: TRPL channel, G-protein, Moesin.
We have found a novel inositide-mediated calcium channel which is activated by light and is necessary for a maintained light excitation. This channel was discovered because of a spontaneous mutation first described by Cosens and Manning, which was designated transient receptor potential (trp) by B. Minke following extensive research that characterized this mutant in detail. The TRP channel of Drosophila turned out to be the founder of a large and diverse family of channel proteins with prime importance for variety of functions in mammalian cells and tissues. We have also found that activation of protein kinase C induces retinal degeneration in a Drosophila mutant and this light induced retinal degeneration can be retarded by voltage-activated Ca2+ channel blockers.
Vered Tzarfati, Ph.D.; Irit Chorna-Ornan, M.Sc; Shahar Frechter, M.Sc.; Shaya Lev, M.Sc.; Moshe Parnas, M.Sc.; Ben Katz, M.Sc.; Elkana Kohn, M.Sc.; Noa Gilad, B.Sc.; Dana Weiss, B.Sc.; Dror Segal, B.Sc.; Natalie Frumkin, B.Sc.; Galit Ankri-Eliahoo, B.Sc.; Shirly Weiss, B.Sc.
Prof. Z. Selinger, Dept. of Biol. Chem., Hebrew Univ.; Prof. Dr. R. Paulsen, Dr. Armin Huber, Univ. of Karlsruhe, Germany; Prof. W.L. Pak, Purdue Univ., W. Lafayette, IN, USA; Prof. D. Hyde, Notre Dame Univ., South Bend, IN, USA; Prof. Jaeseung Yoon, KyungHee University, Korea.
Genetic dissection using classical and molecular genetics: transgenic flies using germline transformation.
Electrophysiology: intracellular dual electrode voltage clamp, patch clamp recordings, ion-selective microelectrodes
Microfluorimetry: fluorescence Ca measurements.
Confocal microscopy autofluorescence of photopigments green fluorescence proteins (GFP) in transgenic flies.
Morphology: light and EM microscopy.
Biochemical techniques: immunoprecipitations, cell fractionations.