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Researchers
  • Prof.  Ittai Ben-Porath
Prof Ittai Ben Porath
 
Lab Interests
 
Our laboratory studies the genetic mechanisms determining tumor growth and metastasis. In order for tumors to grow and to spread throughout the body, cancer cells must acquire various abnormal functions, and this occurs through changes in gene activity. We are interested in the functions acquired by cancer cells and in the genes controlling them.
Our research focuses on two major topics: the factors regulating the differentiation state of breast cancers, and the process of cellular senescence, a major roadblock to tumor development.
 
Molecular regulators of breast cancer differentiation state
Human tumors are highly heterogeneous, differing greatly in their characteristics and their severity. Breast cancers are classified into several subtypes, each displaying distinct traits: certain subtypes display rapid growth and metastasis, causing high mortality rates, while others are typically less aggressive. Highly aggressive tumors are often poorly differentiated, such that they have lost many of the characteristics displayed by the mature tissue and cells in it, and display similarities to normal mammary stem and progenitor cells.
 
Our work is aimed at uncovering the genetic regulators whose activity dictates poor differentiation of breast cancers. Our focus is on regulatory genes known to play a role in the control of normal stem cell function. Analysis of gene expression data derived from many tumors led us to identify previously unknown functions in breast cancer of several such stem cell-associated regulatory genes. One of these is TCF3 (TCF7L1), which encodes a transcription factor of the Wnt pathway-associated TCF/LEF family, and plays key roles in the regulation of epidermal and embryonic stem cell identity. We established that TCF3 is highly expressed in poorly differentiated, aggressive breast cancers and that it promotes the tumor initiation and growth capacity of breast cancer cells. Another gene of interest encodes the chromatin regulator EZH2, which plays an important role in controlling normal stem cell function as a component of the Polycomb complex. In breast cancers EZH2 acts to maintain the gene expression program associated with the highly aggressive and poorly differentiated basal-like subtype, including the expression of progenitor-associated genes. Moreover, EZH2 promotes the expansion of a 'bi-lineage' subpopulation of cells enriched for progenitor traits, which is uniquely found in this tumor type.
 
Our ongoing work is aimed at elucidating the regulatory transcriptional network controlling the differentiation state of these aggressive tumors and the action of specific factors in the context of this network.
3 pic Section of a human basal like breast tumor and Colonies breast cancer cells and Mouse lung seeded with metastatic tumor
Left: Section of a human basal-like breast tumor stained for the lineage markers K14 (red) and K18 (green); bi-lineage cells appear orange. Center: Colonies formed by human breast cancer cells grown in 3D culture and stained for the same markers, indicating ongoing differentiation-like changes.  Right: Mouse lung seeded with metastatic tumor cells, labeled with GFP (green).
 
Cellular senescence as a tumor-suppressive mechanism
In order to form a primary tumor mass cells must overcome barriers that block uncontrolled proliferation. Cellular senescence is a coordinated program that is activated by cells in response to a variety of physiologic stresses, and in response to oncogene activation. Senescent cells cease to proliferate and acquire a variety of distinct morphologic and metabolic traits. Senescence blocks the proliferation of damaged or potentially transformed cells, and is therefore a central tumor suppressive mechanism.
 
Our research is aimed at uncovering the functions of cellular senescence in vivo, in normal and cancerous tissues. Only limited understanding exists of the dynamics of senescent cell formation, the traits of senescent cells, the effects such cells exert on neighboring cells, and the mechanisms responsible for their elimination. We have developed mouse models that enable the induction of cellular senescence in a variety of normal tissues and in developing cancers. These mice allow us to study the process of senescent cell formation, aspects of senescent cell biology, the manner by which such cells affect tissue physiology, and the eventual fate of these cells.
2 pics Cellular senescence induced in the mouse skin and The p19ARF protein is detected in senescent cells in the epidermis 
Left: Cellular senescence induced in the mouse skin, detected by SA-βGal stain (blue). Right: The p19ARF protein (red) is detected in senescent cells in the epidermis.

 
 
Current Lab Members
 
Reba Condiotti

Dror Gal-Kolodkin

Narmen Azazmeh
 
Yael Gabai
 
Hadas Masury
 
Roy Granit

Elisheva Shahar

Tzofia Glickman

Daniela Blumenfeld

Riham Smoom
 
Safa Hirbawi
  
 
Publications

Yosef, R., Pilpel, N., Tokarsky-Amiel, R., Biran, A., Ovadya, Y., Cohen, S., Vadai, E., Dassa, L., Cohen, E., Condiotti, R., Ben-Porath, I.,*  and Krizhanovsky, V.* (2016) Directed Elimination of Senescent Cells by Inhibition of BCL-W and BCL-XL, Nature Comm, doi: 7:10.1038/ncomms11190


Helman, A., Klochendler, A., Azazmeh, N., Gabai, Y., Horwitz, E., Anzi, S., Swisa, A., Condiotti, R., Granit, R.Z., Nevo, Y., Fixler, Y., Shreibman, D., Zamir, A., Tornovsky-Babeay, S., Dai, C., Glaser, B., Powers, A.C., Shapiro, A.M.J., Magnuson, M.A., Dor, Y.*, and Ben-Porath, I.* (2016) p16Ink4a-induced senescence of pancreatic beta cells enhances insulin secretion. Nature Medicine, 22:412-420

Condiotti, R. Guo, W. and Ben-Porath, I. (2014) Evolving views of breast cancer stem cells and their differentiation states. Crit Rev Oncogenesis 19:337-348

Ben-Porath, I. (2014), Active, dormant, or on the path to elimination: what does a senescent cell do? In: Tumor Dormancy, Quiescence and Senescence, Vol. 4, Springer, Editor: M.A. Hayat, Ch1:1-12

Granit, R.Z., Slyper, M., and Ben-Porath, I. (2014) Axes of differentiation in breast cancer: untangling stemness, lineage identity, and the epithelial to mesenchymal transition. Wiley Interdiscip Rev Syst Biol Med 6:93-106.

Tokarsky-Amiel, R., Azazmeh, N., Helman, A., Stein, Y., Maly, A., and Ben-Porath, I. (2013) Controlled induction of p14ARF reveals dynamics of senescent cell formation and retention in vivo. Cancer Res 73:2829-2839


S
lyper, M., Shahar, A., Bar-Ziv, A., Granit, R.Z., Hamburger, T., Maly, B., Peretz, T., and Ben-Porath I. (2012) Control of breast cancer growth and initiation by the stem cell-associated transcription factor TCF3. Cancer Res 72:5613-5624.
 
Granit R.Z., Gabai Y., Hadar T., Karamansha Y., Liberman L., Waldhorn I., Gat-Viks I., Regev A., Maly B., Darash-Yahana M., Peretz T. and Ben-Porath I. (2013) EZH2 promotes a bi-lineage identity in basal-like breast cancer cells. Oncogene 32:3886-3895

Ben-Porath, I., Thomson, MW., Carey, VJ., Ge, R., Bell, GW., Regev, A., and Weinberg, RA. (2008) An embryonic stem cell-like gene expression signature in poorly differentiated aggressive human tumors. Nat. Genet. 40(5):499-507
 
Ben-Porath, I. and Weinberg, R.A. (2005) The signals and pathways activating cellular senescence. Int J Biochem Cell Biol. 37:961-76.
 
Ben-Porath, I and Weinberg, R.A. (2004) When cells get stressed: an integrative view of cellular senescence. Journal of Clinical Investigations 113: 8-13
 
Stewart, S.A.*, Ben-Porath, I.*,  Carey, V.J., O'Connor, B.F., Hahn, W.C. and Weinberg, R.A. (2003) Erosion of the telomeric single-strand overhang at replicative senescence. Nature Genetics 33: 492-496.
 
Masutomi, K., Yu, E.Y., Khurts, S., Ben-Porath, I., Currier, J.L., Metz, G.B., Brooks, M.W., Kaneko, S., Murakami, S., DeCaprio, J.A., Weinberg, R.A., Stewart, S.A. and Hahn, W.C. (2003) Telomerase maintains telomere structure in normal human cells. Cell 114: 241-253.
 
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