The integrity of the genome is constantly challenged by both internal and external DNA damaging agents. DNA damages block transcription and replication, and can lead to cell death. Inaccurate repair of damages leads to mutations and increases the risk of genetic disease and cancer. To understand how DNA damages are formed and how they are repaired in human cells, we have developed state-of-the-art genomic tools that use high-throughput sequencing to map both DNA damage and DNA repair at single-nucleotide resolution.
With these unique genomic tools on-hand, we aim to understand how DNA damage formation and repair occur in the human nucleus, where DNA is packaged into chromatin, and how repair is coordinated with active transcription and replication.