Rhythmic activity is induced by stimulation of sacrocaudal afferent pathways or by bipolar stimulation of the ventromedial medulla (Blivis et al., 2007). Neurochemically induced locomotor like rhythm is initiated by bath-applied NMDA/5HT with or without Dopamine (DA). The sacrocaudal rhythm is selectively produced by bath application of the alpha1-adrenoceptor agonist methoxamine (Gabbay and Lev-Tov, 2004, Cherniak et al. 2011). When required, the experimental bath is divided into different compartments by a Vaseline wall, and neurochemicals are applied to specific segments of the spinal cord (Strauss and Lev-Tov 2003, Blivis et al. 2007, Lev-Tov et al. 2010).
