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Laboratory Techniques

Biochemical: protein purification, chromatography, FPLC, HPLC, electrophoresis, amino acid analysis, sequencing, ultracentrifugation, circular dichroism, Raman spectroscopy, fluorescence, radioactive labeling, pulse chase, DNA/RNA isolation and  labeling, autoradiography, peptide synthesis, solid phase technology, etc.

Molecular: western blotting, immunoprecipitation, RT-PCR, cloning, gene expression, overexpression, site-directed mutagenesis, transfection, transcription, translation, protein and DNA microarrays, receptor assay, receptor signaling, phosphorylation.

Cellular: tissue culture, light microscopy, confocal microscopy, immunohistochemistry, SEM, TEM, transport, exocytosis, neurotransmitter release, proliferation, differentiation, cell death: necrosis, apoptosis, cell adhesion, angiogenesis, bioassay, radioreceptor assay, signal  transduction assays, cell labeling mutagenesis, selection, cloning, sorting, FACS, IMACS, disease models, drug discovery and development.

Tissues: dissection, histology, pharmacological organs preparations (guinea pig ileum, trachea, aortic rings, uterus, heart, brain synaptosomes and slices, neuromuscular preparation, etc.), toxicity, pharmacology, tissue engineering, scaffolds.

Animals: in vivo experiments, small animals surgery, dissection, pharmacological models, transgenic, knockout, drug delivery, pharmacokinetics (ADME assays), behavioral assays, toxicity assays, lethal dose and maximal tolerated dose determination, carcinogenesis.